Genetic Engineering Unlimited !







Taking Care of Your Tomorrow !




Dr Shashi Shekhar


Research Scientist



Research Scientist

Laboratory of Plant Tissue Culture and Biochemical Engineering

Genomic Valley Biotech Limited

4 km Milestone, Beri Chhara Road 

Bahadurgarh, Jhajhar   




Contact: +91-8930068658


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Research Exposure and Experiences

I have completed my Master’s degree in Plant Biochemistry from SHUATS, Allahabad, India in 2007, and enrolled for Ph.D. in 2011 under supervision of Prof. K. Lawrence, SHUATS, Allahabad with Co-Supervision of Prof. N. B. Sarin, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, 110067. My research interests lie in the questions concerned with Genetic Engineering of crop plants for stress alleviation and value addition in crop plants. My research work is primarily focussed on genetic manipulation of crop plants to make them stress tolerance/resistance. For this, I have cloned certain genes of plant’s metabolic pathway which were up regulated during abiotic stress conditions and responsible for the stress tolerance of plants. During my research work I have optimized two different protocols of genetic transformation viz, Centrifugation assisted genetic transformation and Sonication based genetic transformation of Musa Spp. and N. tabacum. Along with the genetic engineering based research work I took certain research work on the enhancement of Artemisinin (Antimalarial Drug) content in a medicinal plant Artemisia annua through cell suspension culture and elicitor based approach.


Award and Fellowship

Ø  The poster entitled “Vaccum infilteration assisted genetic transformation of Banana” (2010), selected under the category of emerging technology in National Conference organized by  Plant Cell Tissue and Organ Culture (PCTOC).

Ø  Project Fellowship from Department of Biotechnology, Govt. of India, 2009-11.

Ø  Project Fellowship from Department of Science and Technology, Govt. of India, 2012-14.

Ø  Senior Research Fellowship from CSIR, Govt of India in 2015.

Ø  Senior Research Fellowship from ICAR, Govt of India in 2016.




1.      Anjana Rustagi ,  Garima Malik,  Samira Chugh,  Shachi Agrawal,  Shashi Shekhar, and Nishakant Pandey (2017) Genetic Manipulation of Litchi for Crop Improvement: Challenges and Possibilities: 301-31; The Lychee Biotechnology: Kumar, M., Kumar, V., Prasad, R., Varma, A. (Eds.) Springer Nature DOI 10.1007/978-981-10-3644-6_11

2.      Shashi Shekhar, Geetika Gambhir, Jasdeep Chatrath Padaria (2017). Bioprospecting of Genes from Microbes for Stress Management in Agricultural Crops: J.S. Singh, G. Seneviratne (eds.), Agro-Environmental Sustainability, 127-147; Springer International DOI 10.1007/978-3-319-49724-2_7

3.      Shashi Shekhar, Anjana Rustagi, Vishnu Bhat, Neera Bhalla Sarin and Kapil Lawrence (2017). Establishment of somatic embryo and plant regeneration via cell suspension cultures of elite Indian cultivars of Banana. (Under review).

4.      Shashi Shekhar, Anjana Rustagi, Neera Bhalla Sarin and Kapil Lawrence (2017). Ectopic expression of stress inducible cytosolic ascorbate peroxidase (cAPX) from peanut enhances multiple abiotic stress tolerance by detoxifying ROS and increasing photosynthetic capacity in Musa sp. cv. Grand Naine. (Under Review)

5.      Shashi Shekhar, Anjana Rustagi, Neera Bhalla Sarin and Kapil Lawrence (2017). Overexpression of salinity induced Cytosolic Ascorbate peroxidase Enhances Resistance of Banana against Fusarium oxysporum f. sp. cubense. (Under Review)

6.      Shashi Shekhar, Kapil Lawrence and Neera Bhalla Sarin (2017). Biosaftey and bioethics of biotechnological research in Indian Context. (Under Review).


7.      Ajeet Kumar Singh, Priyanka O Jaiswal, Shashi Shekhar, Sukriti Goyal, Abhinav Grover, Neera Bhalla Sarin (2017). Enhanced artemisinin production by overexpression of artemisnic aldehyde delta-11(13) reductase in Artemisia annua hairy roots. (Under review).

8.      Anantharaman Perumal, Shailesh Upadhyay, Shashi Shekhar, Kapil Lawrence, Alok Milton Lal, Suresh Murugan (2017). Lipid extraction and production of biodiesel from marine diatom Chaetoceros muelleri isolated from Vellar estuary, Southeast coast of India (Under review).

9.      Anjana Rustagi, Shashi Shekhar, Deepak Kumar, Shalu Jain and Neera Bhalla Sarin (2016). Enhancing Abiotic Stress Tolerance in Banana (Musa spp.) through Genetic Engineering and Biotechnology: S. Mohandas, K.V. Ravishankar (eds.), Banana: Genomics and Transgenic Approaches for Genetic Improvement, Springer Science:181-196; DOI 10.1007/978-981-10-1585-4_12

10.  Neelam Prabha Negi; Divya Shrivastava; Shashi Shekhar; Vinay Sharma; Neera Bhalla Sarin (2016). Gene Pyramiding of AhCuZnSOD and AhcAPX leads to salinity stress tolerance in tobacco. In Vitro Cell. Dev. Biol. Plant; 52: 484. 10.1007/s11627-016-9764-7.

11.  Kumar D, Shekhar S, Bisht S, Kumar V, Varma A (2016) Ectopic Over-expression of Lectin in Transgenic Brassica juncea Plants Exhibit Resistance to Fungal Phytopathogen and Showed Alleviation to Salt and Drought Stress. J Bioengineer & Biomedical Sci 5: 147. doi:10.4172/2155- 9538.1000147

12.  Anjana Rustagi, Shashi Shekhar, Deepak Kumar, Amit Jaiswal, Vishnu Bhat and Neera Bhalla Sarin (2016). Genetic fidelity of rapidly propagated clones from in vitro cultures of an elite Indian Musa (AA) variety Matti. Adv Plants Agric Res 4(3):00141.

13.  Anjana Chaurasia, Shalu Jain, Deepak Kumar , Shashi Shekhar, Mukesh jain, Vishnu Bhat and Neera Bhalla Sarin (2015). High efficiency transformation of Banana [(Musa accuminata cv. Matti (AA)] for enhanced tolerance to salt and drought stress through over-expression of a peanut Salinity-Induced Pathogenesis-Related class 10 protein. Molecular biotechnology, 57(1):27-35. DOI 10.1007/s12033-014-9798-1

14.  Anjana Rustagi, Deepak Kumar, Shashi Shekhar, Mohd Aslam Yusuf, Santosh Misra and Neera Bhalla Sarin (2014). Transgenic Brassica juncea Plants Expressing MsrA1, a Synthetic Cationic Antimicrobial Peptide, Exhibit Resistance to Fungal Phytopathogens. Molecular Biotechnolgy, 56 (6):535-45. DOI 10.1007/s12033-013-9727-8












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