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Dr Shashi Shekhar
Research
Scientist
Research
Scientist
Laboratory of Plant Tissue Culture and Biochemical Engineering
Genomic
Valley Biotech Limited
4 km
Milestone, Beri Chhara Road
Bahadurgarh, Jhajhar
Haryana
124507
India
Contact:
+91-8930068658
Also
Find At:
sshekhar.gvbl@gmail.com
https://www.researchgate.net/profile/Shashi_Shekhar2
https://www.linkedin.com/in/shashi-shekhar-43714962
?
Research Exposure and
Experiences
I have completed my
Master’s degree in Plant Biochemistry from SHUATS, Allahabad, India
in 2007, and enrolled for Ph.D. in 2011 under supervision of Prof.
K. Lawrence, SHUATS, Allahabad with Co-Supervision of Prof. N. B.
Sarin, School of Life Sciences, Jawaharlal Nehru University, New
Delhi, India, 110067. My research interests lie in the questions
concerned with Genetic Engineering of crop plants for stress
alleviation and value addition in crop plants. My research work is
primarily focussed on genetic manipulation of crop plants to make
them stress tolerance/resistance. For this, I have cloned certain
genes of plant’s metabolic pathway which were up regulated during
abiotic stress conditions and responsible for the stress tolerance
of plants. During my research work I have optimized two different
protocols of genetic transformation viz, Centrifugation assisted
genetic transformation and Sonication based genetic transformation
of Musa Spp. and N. tabacum. Along with the genetic
engineering based research work I took certain research work on the
enhancement of Artemisinin (Antimalarial Drug) content in a
medicinal plant Artemisia annua through cell suspension
culture and elicitor based approach.
Award and Fellowship
Ø
The poster
entitled “Vaccum infilteration assisted genetic transformation of
Banana” (2010), selected under the category of emerging technology
in National Conference organized by Plant Cell Tissue and Organ
Culture (PCTOC).
Ø
Project
Fellowship from Department of Biotechnology, Govt. of India,
2009-11.
Ø
Project
Fellowship from Department of Science and Technology, Govt. of
India, 2012-14.
Ø
Senior
Research Fellowship from CSIR, Govt of India in 2015.
Ø
Senior
Research Fellowship from ICAR, Govt of India in 2016.
Publications
1.
Anjana Rustagi ,
Garima Malik,
Samira Chugh,
Shachi Agrawal,
Shashi Shekhar
,
and
Nishakant Pandey (2017)
Genetic Manipulation of Litchi for Crop Improvement: Challenges and
Possibilities:
301-31;
The Lychee Biotechnology
:
Kumar
,
M.,
Kumar,
V.,
Prasad,
R.,
Varma,
A. (Eds.)
Springer
Nature
DOI
10.1007/978-981-10-3644-6_11
2.
Shashi Shekhar,
Geetika Gambhir, Jasdeep Chatrath Padaria (2017).
Bioprospecting of Genes from Microbes for Stress Management in
Agricultural Crops: J.S. Singh, G. Seneviratne (eds.),
Agro-Environmental Sustainability
,
127-147; Springer International DOI 10.1007/978-3-319-49724-2_7
3.
Shashi
Shekhar
, Anjana
Rustagi, Vishnu Bhat, Neera Bhalla Sarin and Kapil Lawrence (2017).
Establishment of somatic embryo and plant regeneration via cell
suspension cultures of elite Indian cultivars of Banana.
(Under
review).
4.
Shashi
Shekhar
, Anjana
Rustagi, Neera Bhalla Sarin and Kapil Lawrence (2017).
Ectopic expression of stress inducible cytosolic ascorbate
peroxidase (cAPX) from peanut enhances multiple abiotic stress
tolerance by detoxifying ROS and increasing photosynthetic capacity
in Musa sp. cv. Grand Naine.
(Under Review)
5.
Shashi
Shekhar
, Anjana
Rustagi, Neera Bhalla Sarin and Kapil Lawrence (2017).
Overexpression of salinity induced Cytosolic Ascorbate peroxidase
Enhances Resistance of Banana against Fusarium oxysporum f.
sp. cubense.
(Under Review)
6.
Shashi
Shekhar,
Kapil
Lawrence and Neera Bhalla Sarin (2017). Biosaftey and
bioethics of biotechnological research in Indian Context.
(Under
Review).
7.
Ajeet Kumar Singh, Priyanka O Jaiswal, Shashi Shekhar,
Sukriti Goyal, Abhinav Grover, Neera Bhalla Sarin (2017).
Enhanced artemisinin production by overexpression of artemisnic
aldehyde delta-11(13) reductase in Artemisia annua hairy
roots.
(Under review).
8.
Anantharaman Perumal, Shailesh Upadhyay, Shashi Shekhar,
Kapil Lawrence, Alok Milton Lal, Suresh Murugan (2017). Lipid
extraction and production of biodiesel from marine diatom
Chaetoceros muelleri
isolated from Vellar estuary, Southeast
coast of India (Under review).
9.
Anjana Rustagi, Shashi Shekhar, Deepak Kumar, Shalu Jain and
Neera Bhalla Sarin (2016). Enhancing Abiotic Stress Tolerance in
Banana (Musa spp.) through Genetic Engineering and
Biotechnology: S. Mohandas, K.V. Ravishankar (eds.),
Banana: Genomics and Transgenic Approaches for Genetic Improvement
,
Springer Science:181-196; DOI 10.1007/978-981-10-1585-4_12
10.
Neelam Prabha Negi; Divya Shrivastava; Shashi Shekhar;
Vinay Sharma; Neera Bhalla Sarin (2016). Gene Pyramiding of
AhCuZnSOD and AhcAPX leads to salinity stress tolerance in tobacco.
In Vitro Cell. Dev. Biol. Plant; 52: 484. 10.1007/s11627-016-9764-7
.
11.
Kumar D, Shekhar S, Bisht S, Kumar V, Varma A (2016)
Ectopic Over-expression of Lectin in Transgenic Brassica juncea
Plants Exhibit Resistance to Fungal Phytopathogen and Showed
Alleviation to Salt and Drought Stress. J Bioengineer & Biomedical
Sci 5: 147. doi:10.4172/2155- 9538.1000147
12.
Anjana Rustagi, Shashi Shekhar, Deepak Kumar, Amit
Jaiswal, Vishnu Bhat and Neera Bhalla Sarin (2016). Genetic fidelity
of rapidly propagated clones from in vitro cultures of an elite
Indian Musa (AA) variety Matti. Adv Plants Agric Res
4(3):00141.
13.
Anjana
Chaurasia, Shalu Jain, Deepak Kumar , Shashi Shekhar, Mukesh
jain, Vishnu Bhat and Neera Bhalla Sarin (2015). High efficiency
transformation of Banana [(Musa accuminata cv. Matti (AA)]
for enhanced tolerance to salt and drought stress through
over-expression of a peanut
Salinity-Induced Pathogenesis-Related
class
10 protein. Molecular biotechnology, 57(1):27-35.
DOI 10.1007/s12033-014-9798-1
14.
Anjana Rustagi, Deepak Kumar, Shashi Shekhar, Mohd
Aslam Yusuf, Santosh Misra and Neera Bhalla Sarin (2014). Transgenic
Brassica juncea Plants Expressing MsrA1, a Synthetic Cationic
Antimicrobial Peptide, Exhibit Resistance to Fungal Phytopathogens.
Molecular Biotechnolgy, 56 (6):535-45. DOI
10.1007/s12033-013-9727-8
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